Lipase is a serine hydrolase enzyme capable of catalyzing the hydrolysis of fatty acid triglycerides, resulting in the production of glycerol and fatty acids. It exhibits its enzymatic activity primarily at the interface between oil and water. Lipase operates effectively within a relatively broad temperature range, typically displaying activity between 30°C and 70°C, with the optimal working temperature falling roughly between 58°C and (35°C). Notably, lipase demonstrates a high degree of pH adaptability, with an effective pH range spanning from 4.0 to 10.0, and the most favorable pH range typically ranging from 6.5 to 7.5. The enzyme's activity is influenced by various metal ions, with some, such as Ca²⁺ and Mg²⁺, exerting a stimulating effect, while certain anions like N³⁻, Cl⁻, and SO₄²⁻ serving as inhibitors. However, the impact of chelating agents like EDTA on the enzyme's activity is relatively minor. Lipase has widespread applications in diverse industries, including leather processing, animal feed production, papermaking, detergents, and the production of biofuels.